Whenever you release the shutter, the mirror that reflects the light coming through your lens up to your viewfinder flips up and out of the way so that light can pass directly through to the sensor. Mirror lockup enables you to flip the mirror up and out of the way ahead of time so that when you release the shutter you won’t have that slight movement that could cause vibrations that might result in blur when using slow shutter speeds. With your camera on a tripod, you would compose your shot through the viewfinder and once your satisfied with your composition and exposure settings, lock the mirror up and then press the shutter release. Of course, if your concerned enough about the vibrations from the mirror, you’ll also want to be using a cable or wireless shutter release or else use the self timer so that you won’t cause vibrations with your finger pressing the shutter release button. Those vibrations would undoubtedly be more of a concern than what would result from the mirror flipping up. It also comes in handy to be able to lock the mirror up and out of the way if you need to clean your sensor.